Effect of a novel piperazine compound on cancer cells

Abstract Many drugs have been developed for anticancer chemotherapy. However, more anti-cancer should be from potential chemicals to circumvent the disadvantages of existing drugs. Most induce apoptosis in cancer cells. This study tested efficiency a new chemical, piperazine derivative 1-[2-(Allylthio) benzoyl]-4-(4-methoxyphenyl) (CB01), on glioblastoma (U87) and cervix (HeLa) CB01 was highly cytotoxic these cells (IC 50S < 50 nM) induced traditional apoptotic symptoms DNA fragmentation nuclear condensation at 40 nM. Western-blot analysis cell lysates revealed that intracellular marker proteins, such as cleaved caspase-3, cytochrome c, Bax, were upregulated CB01-treated Furthermore, increased activities caspase-3 -9, but not caspase-8, observed. Therefore, results suggest can act an chemotherapeutic by stimulating intrinsic mitochondrial signaling pathway cytotoxicity